Abstract Natural Sapajus libidinosus populations are in continuous decline due to fragmentation, habitat loss, and the illegal pet trade. They live in Caatinga scrub forests, which already lost over 50% of their original cover. The lack of studies on S. libidinosus population genetics means that we do not know how they are being affected by this striking habitat loss and other anthropogenic disturbances. Polymorphic markers are not available for the study of S. libidinosus diversity and population genetics. Thus, here we aimed to test the transferability of 14 microsatellite markers to S. libidinosus. These microsatellites were previously isolated from Cebus capucinus (white-faced capuchin), species belonging to the same subfamily (Cebinae) as the study species. We found that six of the tested microsatellite markers (tetra-nucleotide) were cross-amplified in our target species. All loci were polymorphic. The number of alleles varied from 4 to 7, and the expected heterozygosity ranged from 0.588 to 0.869. The microsatellite markers transferred to S. libidinosus and characterised in our study will be valuable tools to evaluate the genetic variability of both wild and captive populations. They will considerably reduce the costs of microsatellite isolations, helping to prioritise currently limited research and conservation budgets in Brazil.

Resumo Introdução A lesão cervical não cariosa (LCNC) é uma doença bucal incidente na atualidade. Objetivo realizar um estudo observacional e descritivo para avaliar fatores de risco associados às lesões cervicais não cariosas, hipersensibilidade dentinária cervical (HDC), recessão gengival (RG) e a relação entre essas condições em estudantes nos últimos anos de graduação em odontologia. Material e método participaram 185 estudantes de odontologia do Ceará com média de 22,7 ± 2,3 anos de idade. Preencheram um formulário do google docs e dois examinadores calibrados realizaram os exames para verificar a presença de LCNC, HDC e RG. Resultado A presença de LCNC foi de 22,7%, a de RG foi de 48,1% e de hipersensibilidade dentinária cervical de 8,6%. O teste qui-quadrado com p < 0,05%, verificou correlação entre a presença de LCNC e a presença de recessão gengival e hipersensibilidade dentinária. Nos alunos do último ano, 25% apresentaram LCNC, dos quais 14% consideravam-se estressados. Não houve associação entre as variáveis bruxismo e estresse (p=0,529), bruxismo e ano do curso da graduação (p=0,716), bem como não foi observada associação de estresse e ano do curso (p = 0,397). Não houve correlação da LCNC e bruxismo, sintomatologia muscular, hábitos parafuncionais e disfunção temporo-mandibular (DTM). Dos alunos que relataram utilizar drogas lícitas e ilícitas, 10,8% apresentaram LCNC. Conclusão A LCNC, RG e HD apresentaram correlação no grupo de estudantes dos dois últimos anos de um curso de graduação em odontologia do Ceará.

Abstract Introduction The non-carious cervical lesion (NCCL) is currently a common oral disease. Objective This observational and descriptive study aimed to assess risk factors associated with non-carious cervical lesions, cervical dentin hypersensitivity (CDH), gingival recession (GR) and the relationship between these conditions in students in the last years of undergraduate dentistry. Material and method One hundred eighty-five dentistry students from a private college in Ceará with an average of 22.7 ± 2.3 years participated by filling out a google docs form. Two calibrated examiners performed the exams to verify the presence of NCCL, CDH and RG. Result The presence of NCCL was 22.7%, GR was 48.1% and cervical dentin hypersensitivity 8.6%. The chi-square test with p < 0.05% verified a correlation between the presence of NCCL and the presence of GR and CDH. 25% of students had NCCL and 14% considered themselves stressed. There was no association between the variables bruxism and stress (p=0.529), bruxism and year of graduation course (p=0.716), as well as no association between stress and year of course (p = 0.397) was observed. There were no correlations between NCCL and bruxism, muscle symptoms, parafunctional habits and temporomandibular dysfunction. 10.8% of students who reported using legal and illegal drugs had NCCL. Conclusion The NCCL, GR and CDH were correlated in the group of students in the last two years of an undergraduate dentistry course in Ceará.

Abstract The solidification of metals and alloys and the resulting microstructures, which as a function of thermal and solutal parameters can evolve as planar, cellular and dendritic, are important from a practical point of view, since they strongly influence the properties and quality of the final product. In many practical situations it is impracticable to develop analytical solutions permitting reliable predictions of microstructural growth during unsteady-state solidification conditions. The Phase Field method has become very popular and effective in modeling complex solid/liquid interfaces due to its ability to simulate the interface kinetics and the formation and evolution of different morphologies along the solidification process. In this work, a numerical analysis of the microstructural evolution during the transient solidification of dilute alloys of the Al-Cu-Si system is developed, which uses a phase-field approach for the simulation of ternary alloys. The phase-field, energy and solute concentration equations were numerically solved for the correspondent ternary system, varying the mesh parameters, temperature and alloy composition. The analysis performed were confronted with existing theoretical models and the results obtained are in agreement with the solidification theory.

RESUMO As características dos materiais utilizados nas embalagens para armazenamento de sementes podem influenciar negativamente no processo de deterioração, acarretando problemas futuros no período de armazenamento e na emergência em campo. Desta forma, objetivou-se avaliar a qualidade fisiológica de sementes de soja, armazenadas em diferentes embalagens com ou sem adição de CO2 (atmosfera modificada) em diferentes períodos e condições ambientais não controladas. Para a realização do experimento foi utilizado um lote de sementes de soja da cultivar SYN 1059 RR. Utilizou-se um delineamento inteiramente casualizado em esquema de parcelas subdivididas, com três tipos de embalagens (embalagem permeável, embalagem hermética dentro da embalagem permeável e embalagem hermética dentro da embalagem permeável com injeção de CO2) na parcela principal e seis períodos de armazenamento (zero, 45, 90, 135, 180 e 225 dias) na subparcela, com quatro repetições para cada tratamento, totalizando 72 unidades experimentais. Foram utilizadas embalagens de papel multifoliado, hermética e hermética com injeção de CO2, armazenadas por 225 dias e avaliação em intervalos regulares de 45 dias. A cada período, as sementes foram submetidas aos testes de grau de umidade, germinação, emergência de plântulas, envelhecimento acelerado e condutividade elétrica. A embalagem hermética, com e sem injeção de CO2, diminui a velocidade da deterioração das sementes de soja. A embalagem hermética permite uma maior qualidade fisiológica das sementes de soja comparativamente à embalagem permeável, no período de armazenamento de até 180 dias sob condições ambientais não controladas. A adição de CO2 no interior de embalagens herméticas favorece a manutenção da qualidade fisiológica de sementes de soja no armazenamento.

ABSTRACT The characteristics of materials used in packages for seed storage may have a negative influence in the deterioration process, causing future problems during the period of storage and in emergence in the field. In this study, the objective was to evaluate the physiological quality of soybean seeds, stored in different packages with and without the addition of CO2 (modified atmosphere), for different periods of storage and in uncontrolled environmental conditions. In order to carry out the experiment, a completely randomized design was used in a split-plot scheme, with three types of packages (permeable, hermetic package inside the permeable package and hermetic package inside the permeable package with CO2 injection) in the main plot and six storage periods (zero, 45, 90, 135, 180 and 225 days) in the subplot, with four repetitions, totaling 72 experimental units. In hermetic packages multi-layer paper was used with or without CO2 injection, stored them for 225 days and performed an assessment at regular intervals of 45 days. At each period, the seeds were submitted to tests for moisture content, germination, seedling emergence, accelerated aging and electrical conductivity. The hermetic package, with and without CO2 injection, decreases the speed of deterioration of soybean seeds. The hermetic package allows a higher physiological quality of soybeans compared to the permeable package, with a storage period of up to 180 days under uncontrolled environmental conditions. The addition of CO2 inside the hermetic packages favors the maintenance of the physiological quality of soybean seeds in storage.

Resumo Embora um grupo de carrapatos moles (Argasidae) associado a anfíbios tenha sido recentemente descoberto nas florestas brasileiras, o parasitismo por esses carrapatos em animais de sangue frio permanece menos comum do que nas espécies de mamíferos e aves. Neste estudo, identificamos carrapatos que foram coletados de sapos capturados em dezembro de 2016 e janeiro de 2017, na cachoeira de Itinguçú (22°54’05” S; 43°53’30” W) no município de Itaguaí, estado do Rio de Janeiro. Os espécimes de carrapatos foram identificados usando uma abordagem morfológica e molecular. No total, doze larvas de carrapatos Ornithodoros foram coletadas de três indivíduos de Rhinella ornata e foram identificadas como Ornithodoros faccinii. Nossos resultados incluem uma maior seqüência mitocondrial 16S rRNA para O. faccinii que suporta sua relação filogenética com Ornithodoros saraivai e relatamos esta espécie de carrapato parasitando sapos Rhinella pela primeira vez no Brasil.

Abstract Although a group of soft ticks (Argasidae) associated with amphibians was recently discovered in Brazilian rainforests, parasitism by these ticks on cold-blooded animals remains less common than on mammal and bird species. In this study, we identified ticks that were collected from toads that had been caught in December 2016 and January 2017, at Itinguçú waterfall (22°54’05” S; 43°53’30” W) in the municipality of Itaguaí, state of Rio de Janeiro. Tick specimens were identified using a morphological and molecular approach. In total, twelve larvae of Ornithodoros ticks were collected from three individuals of Rhinella ornata and were identified as Ornithodoros faccinii. Our results include a longer 16S rRNA mitochondrial sequence for O. faccinii that supports its phylogenetic relatedness to Ornithodoros saraivai, and we report this tick species parasitizing Rhinella toads for the first time in Brazil.

Abstract Dung beetles (Scarabaeinae) interact with resources in different ways and are classified according to resource relocation guilds. Dichotomius geminatus (Coprini) and Deltochilum verruciferum (Canthonini) are two of the most abundant and ecologically important species of the semi-arid region of Brazil, and understanding their behaviour may facilitate the comprehension of strategies associated to competition for resources. The aim of the present study was to investigate the behavioural repertoire of D. geminatus (tunneler) and D. verruciferum (roller), in isolation and controlled setting in the Brazilian semi-arid biome, using carrion as a food resource. Our hypothesis was that, due to the distinct food relocation strategies presented by these species, distinct behaviours would occur involving resource utilization. We also compared the behaviour of the two species and investigated the period of diel activity. Both species were more active during the night, but D. geminatus presented a shorter peak of nocturnal activity when compared to D. verruciferum. Although there was activity during the day, feeding was only observed during the night, for both species. During the periods of inactivity, D. verruciferum commonly went underneath the carrion, remaining still. As the target species of the study are very abundant, the differences in behaviour associated with the distinct relocation guilds may indicate a strategy to avoid direct competition.

Resumo O fator induzível por hipóxia 1 alfa (HIF-1α) e o fator de crescimento endotelial vascular (VEGF) são proteínas que estimulam a proliferação e a migração de células endoteliais. Estas proteínas têm sido descritas em muitas condições patológicas e inflamatórias, mas o envolvimento dessas no desenvolvimento de periodontite não foi completamente investigado. Este estudo comparou a expressão imunohistoquímica destas proteínas, envolvidas na angiogênese e hipóxia, na doença periodontal e em tecidos gengivais saudáveis por meio de contagem de células inflamatórias e endoteliais imunomarcadas. As amostras de tecido gengival foram divididas da seguinte forma: 30 amostras com periodontite crônica, 30 com gengivite crônica e 30 de gengiva saudável. Os resultados foram analisados estatisticamente pelos testes de Kruskal-Wallis e Mann-Whitney (p=0.01). As células inflamatórias e endoteliais foram positivas para estas proteínas. A porcentagem média de células positivas para HIF-1α foi de 39,6% nos casos de periodontite, 22,0% nos casos de gengivite e de 0,9% no grupo de gengiva saudável (p = 0,001). A porcentagem média de células imunopositivas para o VEGF foi de 68,7% nos casos de periodontite, 66,1% nos casos de gengivite, e 19,2% em espécimes gengivais saudáveis (p<0,001). Correlação significativa entre o VEGF e HIF-1α foi observada em gengival. A expressão elevada do HIF-1α e VEGF em periodontite, comparada a gengivite e gengiva saudável, sugere ativação da via do HIF-1α, na doença periodontal avançada. A correlação entre o HIF-1α e expressão de VEGF na gengiva saudável, sugere uma função fisiológica para estas proteínas em condições de homeostase. Na doença periodontal, a expressão de HIF-1α e VEGF pode ser regulada por outros fatores, além da hipóxia, tais como endotoxinas bacterianas e citocinas inflamatórias.

Abstract Hypoxia-inducible factor 1 alpha (HIF-1α) and vascular endothelial growth factor (VEGF) are proteins that stimulate the proliferation and migration of endothelial cells. These proteins have been described in many pathologic and inflammatory conditions, but their involvement in the development of periodontitis has not been thoroughly investigated. This study compared the immunohistochemical expression of these proteins, involved in angiogenesis and hypoxia, by imunnostained inflammatory and endothelial cells in periodontal disease and healthy gingival tissues. Gingival tissue samples were divided as follows: 30 samples with chronic periodontitis, 30 with chronic gingivitis, and 30 of healthy gingiva. Results were analyzed statistically by the Kruskal-Wallis, Mann-Whitney and Spearman correlation tests (p=0.01). Inflammatory and endothelial cells were found to express these proteins. Periodontitis showed median percentage of HIF-1α-positive cells of 39.6%, 22.0% in cases of gingivitis and 0.9% in the healthy gingiva group (p=0.001). For VEGF, median percentage of immunopositive cells was 68.7% for periodontitis, 66.1% in cases for gingivitis, and 19.2% for healthy gingival specimens (p<0.001). Significant correlation between VEGF and HIF-1α was also observed in healthy gingiva (p<0.001).The increased expression of HIF-1αα and VEGF in periodontitis, compared to gingivitis and healthy gingiva, suggests possible activation of the HIF-1α pathway in advanced periodontal disease. The correlation between HIF-1α and VEGF expression in healthy gingiva suggests a physiological function for these proteins in conditions of homeostasis. In periodontal disease, HIF-1 and VEGF expression may be regulated by other factors, in addition to hypoxia, such as bacterial endotoxins and inflammatory cytokines.